About this manual. This manual describes the operation of the ÄKTA™FPLC™ system. System description, system maintenance and trouble-shooting are also. filter. Sample pump. Buffer select valve (V6). Injection valve (V1). Reverse . Manual. Flowpath. B1 or B2. BufferValveB. Pump A. Pump B. Common inlet. A2. A1. About this manual This manual describes the operation of ÄKTA purifier: Evaluating the UV response Calculate the UV response ratios in the following.

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Mix, using a 25 ml pipette, 25 ml of the buffer stock solution, 25 ml of the acid and 50 ml of water. There’s no available product features to display for this product variant. BufferPrep eliminates the time consuming manual buffer preparation and titration usually needed for every pH change in chromatography. The flow must be off purfier starting the filling procedure. Calibrate in System Control.

All repairs should be done by personnel authorized by by GE Healthcare. To calculate the new delay volume see Section 8.

Some packing lists are included in the boxes. All documentation about the run is stored here, e.

When scouting over a broad pH range, fine tuning is less beneficial. The table below shows the tubing dimensions installed at the factory and their location in the system.

Run Data The Run Data pane displays the current values for selected run parameters. When assembling the on-line filter, tighten the filter body by hand only. Only use tubing supplied by GE Healthcare to make sure that the pressure specifications of the tubing are fulfilled.


ÄKTApurifier – GE Healthcare Life Sciences

Check if there is too low concentration of buffer components causing low buffering mankal. For more information on: Open refers to the Open command in the File menu. Check the tubing connectors. Be sure not to proceed until the instructions are clearly understood and all stated conditions are met.

Superloop 10 ml and Superloop 50 ml must not be used at pressures above 4 MPa 40bar, psi. The following table shows which technique is recommended for different sample volumes. Attach the column holder enclosed in Box above the UV cell.

A mm long i. Specified for 10 mm flow cell 2.

When the system configuration after the UV cell has been changed, for example by changing a component or tubing, the delay volume might change as well. Increase the acid concentration by the same factor as the buffer concentration. Two small akha on the cover locate in holes at the front and rear of the cell holder. D can have values between 0 and infinity, where larger values have a greater effect and a value of 0 has no effect. Starting the computer Turn on the computer, the display and the printer according to the instructions in the manufacturer manuals.

It will only be activated during sample application and wash-out of unbound sample. Chemical influences are time and pressure dependent.

This flow restrictor is used when a pH cell optional is fitted in the system, or when a low pressure column is used. In the Save As dialog, enter a name. Only the pages set in the Start Protocol for the method will appear during the initialization. Sample loss during tube change can be eliminated by using drop synchronisation, or by using a built-in accumulator for intermediate liquid storage between tube change.


In each dialog, select the appropriate parameter values. It is used as a bypass when the column is removed from ourifier separation unit, e. Use it as follows: This allows for binary gradients with high pressure mixing. Here you can verify and fine manuao the method before you proceed.

They can also be used for changing buffer during the method run. The table below lists the theoretical volumes of a number of components and tubing. Run Setup in Method Editor. Wipe the surface regularly with a damp cloth. In a domestic environmentit may cause radio interference, in which case the user may be required to take suitable measures. Both ends should now be in the flask 1. The computer should be installed and used according to the instructions provided by the manufacturer of the computer.

Correct gradient formation is tested by producing a linear gradient and a series of concentration steps of acetone.

ÄKTApurifier User Guide – GE Healthcare Life Sciences

For scouting runs If the sample volume is to be varied automatically in a series of scouting runs, mxnual of the following techniques can be used: Check that the pH is not set too far from the pKa of the buffer components. A method file contains a series of instructions for controlling a run. You puurifier a page by clicking the respective tab at the top of the window. PressLevel is the maximum allowed pressure.

Contact your local GE Healthcare representative for the most current information.